Mange tak!

Bare jeg havde sådan et filter som de har der, det ville nok gøre
det lidt nemmere at undgå kontamination. Jeg er gået igang med
at bygge en glovebox som jeg håber kan være behjælpelig til det samme...

Desværre mangler jeg en trykkoger, så jeg bruger en almindelig gryde.
Jeg fornemmer at det er den største kilde til kontamination.

For this, I am of the thinking that SIMPLE IS BEST. My liquid culture containers are simple jars, with a regular old metal lid and ring, with a single hole punched at the top. The hole should be big enough to accomodate your needle, with a little room to spare so that a vaccum is not created later when aspirating solution. The hole is covered with a piece of micropore tape, and then covered again either with a coffee filter and rubber band, or a piece of aluminum foil loosely crumpled, to act as a dust cover.

The master culture. Notice its very thick! Obviously my little micropore'd hole provides all the gas exchange you need. You'll also note a few dark specs, that's leftover pieces of the stem. It kind of dissolves and breaks up to an extent. It turns dark blue/grey, because you bruised it for sure when poking it with a needle!

When I aspirate, I take an alcohol soaked cotton swab, and swab right over the piece of micropore tape. I then stick the needle right through the tape, and fill up my syringe. I quickly cap the syringe, and quickly replace the soggy piece of micropore tape with a fresh piece. You can certainly use any number of the fancy LC container teks out there, but it doesn't get much simpler than a hole with a piece of tape on it kids.